Development and validation of a spectrofluorimetric method for the estimation of Tenofovir in bulk and formulation
University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India.
Research Article
World Journal of Advanced Engineering Technology and Sciences, 2023, 08(01), 231–238.
Article DOI: 10.30574/wjaets.2023.8.1.0033
Publication history:
Received on 22 December 2022; revised on 30 January 2023; accepted on 07 February 2023
Abstract:
A simple, accurate, precise, sensitive and cost-effective spectrofluorimetric method was developed and validated for the estimation of Tenofovir in bulk and formulation. The relative fluorescence intensity of Tenofovir was measured in Distilled Water: Methanol (40:60%v/v) at an excitation wavelength of 374 nm and an emission wavelength of 409 nm. Proposed method was found to be linear over the range of 50 to 1000 ng/ml with correlation coefficient 0.999. Proposed method was validated using different analytical method validation parameters viz. Accuracy, precision, LOD, LOQ, robustness and ruggedness using QC standards as per the ICH guidelines. The percentage recovery was found to be 100.29 % and percentage RSD values were found to be less than 2 for accuracy and precision studies. The detection and quantification limits for the proposed method were found to be 3.009ng/ml and 9.120ng/ml, respectively. A simple, accurate, precise, sensitive yet cost-effective spectrofluorimetric method was developed for the estimation of Tenofovir in bulk and formulation. The said spectrofluorimetric method was found to be economic as it comprises water as a solvent.
Keywords:
Spectrofluorimetry; Tenofovir; Validation; Excitation; Emission
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0