Influence of various substrate treatment methods on yield and biological efficiency of Pleurotus florida mushroom

Due to their excellent flavour and taste, Pleurotus species, which flourish in temperate and subtropical environments, are well-liked among edible mushrooms. Oyster mushrooms could be grown on a variety of substrates due to their powerful enzymatic properties. Pleurotus sp. grows more easily, more profitably, and more nutrient-densely on a variety of organic waste raw materials and environmental factors. Sterilization of the substrate is one of the most crucial steps in oyster mushroom cultivation. There have been numerous reports of contaminated mushroom substrate. Suitable sterilization techniques must be used on the substrates. There are several treatments for mushroom substrate that can be used to manage the frequent moulds that infest edible mushrooms. Sterilization of the substrate is necessary to get rid of pathogenic and rival microorganisms and to encourage the mycelial growth of the desired mushroom species. The goal of the current study was to identify the best treatment strategy for the chosen substrates. For substrate treatment for Pleurotus florida cultivation, hot water, boiling water, steam sterilization in an autoclave, and chemical treatment methods were used. It was observed that the steam sterilization method was superior to other methods as it supported relatively short spawn run duration, amazing yield, and high biological efficiency.