Synthesis, Characterization and Antimicrobial Analysis of 1-(2-Nitrophenylazo)-2-Naphthol

Scientists are searching for efficient antibiotic medications to treat pathogenic microorganism-caused illnesses since these microbes have become a major medical threat because of the illnesses and infections they can induce in their hosts. Azo naphthol dyes are often vividly coloured substances with a variety of industrial applications. Lately, they have demonstrated efficacy against specific pathogenic microbe strains. In this study, 2-nitroaniline and 2-naphthol underwent an azo coupling reaction to create 1-(2-nitrophenylazo)-2-naphthol azo dye. The compound was identified using the information gleaned from the IR, NMR, and UV-vis spectra. The results of the antimicrobial investigation demonstrated that the azo compound has the ability to suppress certain types of bacteria, including Escherichia coli and Staphylococcus aureus, as well as fungus, such as Aspergillus sp. The compound concentration of 150 g/mL was resisted by the fungi but inhibited the bacteria at the range of 0.5 – 0.8 mm while 50 g/mL and 100 g/mL concentrations were resisted across by the organisms. Then, 200 g/mL, raw, and 30 g/mL control drugs (Oflocitoxin and Augmentin) concentrations inhibited the organisms at the range of 0.5 – 1.2 mm, 1.1 – 1.7 mm, 1.6 – 24 mm (1.6 – 15 mm and 2.5 – 24 mm), respectively. The minimum inhibitory concentration of the compound was estimated at 150 g/mL for the bacteria, and 200 g/mL for the fungus used. The results showed that the compound can be used as an antimicrobial agent for a lot of industrial and medical applications.